Ophthalmology
Volume 112, Issue 3 , Pages 470-477 , March 2005

Outcomes and DNA analysis of ex vivo expanded stem cell allograft for ocular surface reconstruction

  • Sheraz M. Daya, FACP, FACS

      Affiliations

    • Corneo-Plastic Unit, Queen Victoria Hospital, East Grinstead, United Kingdom
    • Corresponding Author InformationCorrespondence to Sheraz M. Daya, MD, FACP, FACS, Director, Corneo-Plastic Unit and Eye Bank, Queen Victoria Hospital, East Grinstead, West Sussex, RH19 3DZ, United Kingdom
    • ,
  • Adam Watson, FRANZCO

      Affiliations

    • Corneo-Plastic Unit, Queen Victoria Hospital, East Grinstead, United Kingdom
    • ,
  • Justin R. Sharpe, PhD

      Affiliations

    • Blond McIndoe Centre, Queen Victoria Hospital, East Grinstead, United Kingdom
    • ,
  • Osama Giledi, FRCS

      Affiliations

    • Corneo-Plastic Unit, Queen Victoria Hospital, East Grinstead, United Kingdom
    • ,
  • Andrea Rowe

      Affiliations

    • East Grinstead Eye Bank, Queen Victoria Hospital, East Grinstead, United Kingdom
    • ,
  • Robin Martin, PhD

      Affiliations

    • Blond McIndoe Centre, Queen Victoria Hospital, East Grinstead, United Kingdom
    • ,
  • S. Elizabeth James, PhD

      Affiliations

    • Blond McIndoe Centre, Queen Victoria Hospital, East Grinstead, United Kingdom

Received 9 December 2003 ,Accepted 8 September 2004.

References 

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  2. Holland EJ , Schwartz GS . Changing concepts in the management of severe ocular surface disease over twenty-five years . Cornea . 2000;19:688–698
  3. Tsubota K , Satake Y , Kaido M , et al.  Treatment of severe ocular-surface disorders with corneal epithelial stem-cell transplantation . N Engl J Med . 1999;340:1697–1703
  4. Daya SM , Ilari L . Living related conjunctival limbal allograft for the treatment of stem cell deficiency . Ophthalmology . 2001;108:126–133
  5. Ilari L , Daya SM . Long-term outcomes of keratolimbal allograft for the treatment of severe ocular surface disorders . Ophthalmology . 2002;109:1278–1284
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  7. Rao SK , Rajagopal R , Sitalakshmi G , Padmanabhan P . Limbal allografting from related live donors for corneal surface reconstruction . Ophthalmology . 1999;106:822–828
  8. Pellegrini G , Traverso CE , Franzi AT , et al.  Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium . Lancet . 1997;349:990–993
  9. Tsai RJ , Li LM , Chen JK . Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells . N Engl J Med . 2000;343:86–93
  10. Schwab IR , Reyes M , Isseroff RR . Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease . Cornea . 2000;19:421–426
  11. Grueterich M , Tseng SC . Human limbal progenitor cells expanded on intact amniotic membrane ex vivo . Arch Ophthalmol . 2002;120:783–790
  12. James SE , Rowe A , Ilari L , et al.  The potential for eye bank limbal rings to generate cultured corneal epithelial allografts . Cornea . 2001;20:488–494
  13. Rheinwald JG , Green H . Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells . Cell . 1975;6:331–343
  14. Tsubota K , Satake Y , Ohyama M , et al.  Surgical reconstruction of the ocular surface in advanced ocular cicatricial pemphigoid and Stevens-Johnson syndrome . Am J Ophthalmol . 1996;122:38–52
  15. Kasper M , Moll R , Stosiek P , Karsten U . Patterns of cytokeratin and vimentin expression in the human eye . Histochemistry . 1988;89:369–377
  16. Barnard Z , Apel AJ , Harkin DG . Phenotypic analyses of limbal epithelial cell cultures derived from donor corneoscleral rims . Clin Experiment Ophthalmol . 2001;29:138–142
  17. Schermer A , Galvin S , Sun TT . Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells . J Cell Biol . 1986;103:49–62
  18. Zieske JD , Bukusoglu G , Yankauckas MA , et al.  Alpha-enolase is restricted to basal cells of stratified squamous epithelium . Dev Biol . 1992;151:18–26
  19. Kasper M . Patterns of cytokeratins and vimentin in guinea pig and mouse eye tissue: evidence for regional variations in intermediate filament expression in limbal epithelium . Acta Histochem . 1992;93:319–332
  20. Krenzer KL , Freddo TF . Cytokeratin expression in normal human bulbar conjunctiva obtained by impression cytology . Invest Ophthalmol Vis Sci . 1997;38:142–152
  21. Grueterich M , Espana EM , Touhami A , et al.  Phenotypic study of a case with successful transplantation of ex vivo expanded human limbal epithelium for unilateral total limbal stem cell deficiency . Ophthalmology . 2002;109:1547–1552
  22. Koizumi N , Inatomi T , Suzuki T , et al.  Cultivated corneal epithelial stem cell transplantation in ocular surface disorders . Ophthalmology . 2001;108:1569–1574
  23. Tseng SC , Meller D , Anderson DF , et al.  Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane for treating corneal diseases with total limbal stem cell deficiency . Adv Exp Med Biol . 2002;506:1323–1334
  24. Shimazaki J , Aiba M , Goto E , et al.  Transplantation of human limbal epithelium cultivated on amniotic membrane for the treatment of severe ocular surface disorders . Ophthalmology . 2002;109:1285–1290
  25. Dua HS . The conjunctiva in corneal epithelial wound healing . Br J Ophthalmol . 1998;82:1407–1411

 Manuscript no. 230850.

 Dr Martin's current affiliation is Smith and Nephew, York, United Kingdom.

 Funded by the East Grinstead Medical Research Trust, East Grinstead, United Kingdom; the John Ellerman Foundation, London, United Kingdom; the Band Trust, London, United Kingdom; and the Eye Bank Association of America, Washington, DC.

 Presented in part at: American Academy of Ophthalmology meeting, November, 2003; Anaheim, California.

PII: S0161-6420(04)01496-4

doi: 10.1016/j.ophtha.2004.09.023

Ophthalmology
Volume 112, Issue 3 , Pages 470-477 , March 2005

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