Corneal Ectasia After Excimer Laser Keratorefractive Surgery: Histopathology, Ultrastructure, and Pathophysiology
Presented at the Annual Meeting of the American Academy of Ophthalmology, Las Vegas, Nevada, November 13, 2006.
Received 5 March 2008; received in revised form 4 June 2008; accepted 5 June 2008. published online 11 August 2008.
Purpose
To evaluate the histopathology and ultrastructure of corneas developing ectasia after LASIK or photorefractive keratectomy (PRK).
Design
Retrospective case series.
Participants
Thirteen specimens from 12 patients undergoing corneal transplantation for progressive ectasia after LASIK (12 specimens) or PRK (1 specimen) were obtained for histopathologic and ultrastructural evaluation.
Methods
All 13 ectatic corneas were submitted in formalin for light microscopy. Nine specimens were bisected, and the second half was placed in 2.5% glutaraldehyde for transmission electron microscopy (TEM).
Main Outcome Measures
Corneal histopathology, ultrastructure, and pathophysiology.
Results
Light microscopy of the post-LASIK specimens showed corneal epithelial hypoplasia and occasional foci of epithelial hyperplasia, Bowman's layer breaks, a normal stromal thickness of the LASIK flap, a normal thickness of the hypocellular primitive stromal scar, a thinned residual stromal bed (RSB), and larger than normal artifacteous interlamellar clefts in the RSB of the ectatic region. The post-PRK specimen showed similar findings with the addition of a thinned hypercellular fibrotic stromal scar. TEM showed thinning of the collagen lamellae and loss of lamellar number in the RSB of post-LASIK ectasia corneas or throughout the entire corneal stromal bed in the post-PRK ectasia cornea, with the posterior aspect of the corneal stroma being most affected.
Conclusions
Histopathologic and ultrastructural studies suggest that interlamellar and interfibrillar biomechanical slippage occurs when the cornea becomes ectatic after LASIK or PRK in the postoperative stress-bearing regions of the corneal stroma. This 2-phase chronic biomechanical failure process is similar to that seen in keratoconus. Composite sciences classify this chronic biomechanical failure process as interfiber fracture.
Financial Disclosure(s)
The authors have no proprietary or commercial interest in any materials discussed in this article.
Available online: August 9, 2008.
1Emory Eye Center, Emory University School of Medicine, Atlanta, Georgia
2Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, Florida
3Department of Ophthalmology, Ruprecht-Karls University, Heidelberg, Germany
Correspondence: Henry F. Edelhauser, PhD, Emory Eye Center, 1365-B Clifton Road, NE, Atlanta, GA 30322
Manuscript no. 2008-291.
Financial Disclosure(s): The authors have no proprietary or commercial interests in any materials discussed in this article.
Supported in part by National Eye Institute grants: EY-00933, P30-EY06360, T32-EY07092, an unrestricted departmental grant from Research to Prevent Blindness, and the ASCRS Foundation's Research Grant Program.
ABSTRACT