In Vivo Confocal Microscopic Findings of Corneal Wound Healing after Corneal Epithelial Debridement in Diabetic Vitrectomy
Presented at: Association for Research in Vision and Ophthalmology Annual Meeting, April to May 2008, Fort Lauderdale, Florida.
Received 18 September 2008; received in revised form 2 January 2009; accepted 6 January 2009. published online 27 April 2009.
Purpose
To study healing of corneal wounds using in vivo confocal microscopy in patients who received corneal epithelial debridement during pars plana vitrectomy for proliferative diabetic retinopathy and to investigate risk factors for delayed healing.
Design
Prospective, observational case series.
Participants
Forty-four eyes of 40 patients were enrolled.
Methods
In vivo confocal microscopy was used to evaluate selected images of the corneal basal and apical surface epithelial cells and subbasal nerves before surgery, weekly for the first month, and at 3 and 6 months after surgery. Slit-lamp biomicroscopy was carried out at the same time. Multiple linear regression analysis of selected potential risk factors was performed to investigate the main determinants of delayed corneal healing.
Main Outcome Measures
Healing rate of corneal epithelial cells and subbasal nerves and factors influencing the healing.
Results
By slit-lamp biomicroscopy, corneal epithelial defects were found in 22.8% of eyes at 2 weeks and in 5.4% at 1 month after surgery. In vivo confocal microscopy demonstrated incomplete healing of basal epithelial cells in 72.1%, 15.2%, and 0% of eyes and incomplete healing of surface apical epithelial cells in 81.1%, 9.1%, and 0% of eyes at 1, 3, and 6 months after surgery. The percentage of subbasal nerves regaining preoperative appearance was 0%, 6.8%, and 89.3% at 1, 3, and 6 months after surgery. Regression analysis revealed infusion of silicone oil (P = 0.020) and C3F8 (P = 0.017) resulted in delayed healing by slit-lamp biomicroscopy; age (P = 0.028), diabetic treatment regimen (P = 0.014), and scleral buckling (P = 0.001) correlated with delayed recovery of basal cells by in vivo confocal microscopy. The latter 2 factors also were related to delayed reconformation of apical cells (P = 0.011 and 0.004, respectively). Neither healing of apical and basal cells showed a significant correlation to findings by slit-lamp biomicroscopy (r = 0.19 and 0.09).
Conclusions
Healing of corneal epithelial wounds in diabetic eyes is slow. Both the basal and apical epithelial layers were involved in the slow healing process. Age, diabetic treatment regimen, and several intraoperative factors may alter healing rates.
Financial Disclosure(s)
The author(s) have no proprietary or commercial interest in any materials discussed in this article.
Available online: April 27, 2009.
1Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan
2Center of Corneal Tissue Engineering and Stem Cell Biology, National Taiwan University Hospital, Taipei, Taiwan
3Department of Veterinary Medicine, College of Bio-Resource and Agriculture, National Taiwan University, Taipei, Taiwan
Correspondence: Chung-May Yang, MD, Department of Ophthalmology, National Taiwan University Hospital, 7, Chung-Shan South Road, Taipei, Taiwan
Manuscript no. 2008-1127.
Financial Disclosure(s): The author(s) have no proprietary or commercial interest in any materials discussed in this article.
Supported in part by the Department of Medical Research at the National Taiwan University Hospital, Taipei, Taiwan.
ABSTRACT